Journal: Heliyon

Article Title: High-fat and high-carbohydrate diets worsen the mouse brain susceptibility to damage produced by enterohemorrhagic Escherichia coli Shiga toxin 2

doi: 10.1016/j.heliyon.2024.e39871

Figure Lengend Snippet: Changes in microvasculature lectin fluorescence pattern. A: the murine VA-VL brain area studied; B: micrograph shows the microvasculature profile in the ND + veh control group; C: ND + Stx2 treatment; D: HF + veh treatment; E: HF + Stx2 treatment; F: HC + veh treatment; G: HC + Sxt2 treatment; H: HF + HC + veh; I: HF + HC + Stx2; J: negative control by not adding Lycopersicum esculentum lectins; K: number of lectin-immunopositive particles; L: size of microvessels (μm2); M: area occupied by microvessels (%); arrow in B: a conserve microvessel; arrowheads in E: fragmented microvessels. Data were analyzed by one-way ANOVA and Bonferroni post hoc test, p = 0.0001, n = 5. Scale bar in J applies to all panels.

Article Snippet: In all assays, statistical analyses were conducted using one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test (GraphPad Prism 8, GraphPad Software, Inc., San Diego, CA, USA) between i.v. treatments (control and Stx2).

Techniques: Fluorescence, Control, Negative Control

Journal: Heliyon

Article Title: High-fat and high-carbohydrate diets worsen the mouse brain susceptibility to damage produced by enterohemorrhagic Escherichia coli Shiga toxin 2

doi: 10.1016/j.heliyon.2024.e39871

Figure Lengend Snippet: Changes in astrocyte GFAP expression. A: the murine internal capsule brain area studied; B: micrograph shows the astrocyte expression of GFAP in the ND + veh control group; C: ND + Stx2 treatment; D: HF + veh treatment; E: HF + Stx2 treatment; F: HC + veh treatment; G: HC + Stx2 treatment; H: HF + HC + veh treatment; I: HF + HC + Stx2 treatment; J: negative control by omitting primary antibody; K: quantification of GFAP expression levels in all treatments. Data were analyzed by one-way ANOVA and Bonferroni post hoc test, p = 0.0001, n = 5. Scale bar in J applies to all panels.

Article Snippet: In all assays, statistical analyses were conducted using one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test (GraphPad Prism 8, GraphPad Software, Inc., San Diego, CA, USA) between i.v. treatments (control and Stx2).

Techniques: Expressing, Control, Negative Control

Journal: Heliyon

Article Title: High-fat and high-carbohydrate diets worsen the mouse brain susceptibility to damage produced by enterohemorrhagic Escherichia coli Shiga toxin 2

doi: 10.1016/j.heliyon.2024.e39871

Figure Lengend Snippet: Changes in the neuron NeuN fluorescence patterns. A: the murine VA-VL brain area studied; B: micrograph shows NeuN localization in the ND + veh control group; C: ND + Stx2 treatment; D: HF + veh treatment; E: HF + Stx2 treatment; F: HC + veh treatment; G: HC + Stx2 treatment; H: HF + HC + veh treatment; I: HF + HC + Stx2 treatment; J: negative control by omitting primary antibody; K: quantification of nuclei with abnormal phenotypes in all treatments. Arrowhead in B: normal phenotype; arrow in I: abnormal phenotype. Data were analyzed by one-way ANOVA and Bonferroni post hoc test, p = 0.0001, n = 5. Scale bar in J applies to all panels.

Article Snippet: In all assays, statistical analyses were conducted using one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test (GraphPad Prism 8, GraphPad Software, Inc., San Diego, CA, USA) between i.v. treatments (control and Stx2).

Techniques: Fluorescence, Control, Negative Control

Journal: Heliyon

Article Title: High-fat and high-carbohydrate diets worsen the mouse brain susceptibility to damage produced by enterohemorrhagic Escherichia coli Shiga toxin 2

doi: 10.1016/j.heliyon.2024.e39871

Figure Lengend Snippet: Changes in Iba1 expression and number of Iba1-positive cells. A: the murine VA-VL brain area studied; B: micrograph shows the expression of Iba1 and the number of Iba1-positive cells in the ND + veh control group; C: ND + Stx2 treatment; D: HF + veh treatment; E: HF + Stx2 treatment; F: HC + veh treatment; G: HC + Stx2 treatment; H: HF + HC + veh treatment; I: HF + HC + Stx2 treatment; J: negative control by omitting primary antibody; K: quantification of Iba1 expression levels in all treatments. Data were analyzed by one-way ANOVA and Bonferroni post hoc test, p = 0.0001, n = 5. Scale bar in J applies to all panels.

Article Snippet: In all assays, statistical analyses were conducted using one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test (GraphPad Prism 8, GraphPad Software, Inc., San Diego, CA, USA) between i.v. treatments (control and Stx2).

Techniques: Expressing, Control, Negative Control

Journal: Heliyon

Article Title: High-fat and high-carbohydrate diets worsen the mouse brain susceptibility to damage produced by enterohemorrhagic Escherichia coli Shiga toxin 2

doi: 10.1016/j.heliyon.2024.e39871

Figure Lengend Snippet: Changes in MBP expression. A: the murine internal capsule brain area studied; B: micrograph shows the expression of MBP in the ND + veh control group; C: ND + Stx2 treatment; D: HF + veh treatment; E: HF + Stx2 treatment; F: HC + veh treatment; G: HC + Stx2 treatment; H: HF + HC + veh treatment; I: HF + HC + Stx2 treatment; J: negative control by omitting primary antibody; K: quantification of MBP expression levels in all treatments. Data were analyzed by one-way ANOVA and Bonferroni post hoc test, p < 0.005, n = 5. Scale bar in J applies to all panels.

Article Snippet: In all assays, statistical analyses were conducted using one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test (GraphPad Prism 8, GraphPad Software, Inc., San Diego, CA, USA) between i.v. treatments (control and Stx2).

Techniques: Expressing, Control, Negative Control

Journal: Heliyon

Article Title: High-fat and high-carbohydrate diets worsen the mouse brain susceptibility to damage produced by enterohemorrhagic Escherichia coli Shiga toxin 2

doi: 10.1016/j.heliyon.2024.e39871

Figure Lengend Snippet: Changes in mouse motor behavior. A: design of the test device; B: columns indicate the different treatments. Data were analyzed by one-way ANOVA and Bonferroni post hoc test, p = 0.0001, n = 10.

Article Snippet: In all assays, statistical analyses were conducted using one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test (GraphPad Prism 8, GraphPad Software, Inc., San Diego, CA, USA) between i.v. treatments (control and Stx2).

Techniques:

Journal: Heliyon

Article Title: High-fat and high-carbohydrate diets worsen the mouse brain susceptibility to damage produced by enterohemorrhagic Escherichia coli Shiga toxin 2

doi: 10.1016/j.heliyon.2024.e39871

Figure Lengend Snippet: Changes in mouse sensitivity. A: design of the test device; B: columns indicate the different treatments. Data were analyzed by one-way ANOVA and Bonferroni post hoc test, p = 0.0001, n = 10.

Article Snippet: In all assays, statistical analyses were conducted using one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test (GraphPad Prism 8, GraphPad Software, Inc., San Diego, CA, USA) between i.v. treatments (control and Stx2).

Techniques: